Physiology

Methods of Hormone Radioimmunoassay by Bernard M. Jaffe

By Bernard M. Jaffe

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6 % (n = 3), 6 8 . 1 ± 1 3 . 7 % (n = 255), a n d 6 8 . 9 ± 6 . 6 % (n = 12), r e s p e c t i v e l y . T h e r e f o r e , w e o r d i n a r i l y follow r e c o v e r y of j u s t 3 H - P G E 1 a n d c o r r e c t r a d i o i m m u n o a s s a y d e t e r m i n a t i o n s of all t h r e e p r o s t a g l a n d i n g r o u p s b y this figure. If w e a r e p a r t i c u l a r l y i n t e r e s t e d in d e t e r m i n a t i o n s of P G A or P G F , w e find it a p p r o p r i a t e to a d d t h a t p a r t i c u l a r l a b e l to p l a s m a for r e c o v e r y c o r r e c t i o n .

Prostaglandins 1, 407-423. 2. , and Elder, M. G (1977). Radioimmunoassay of 15-keto-prostaglandin E 2 in peripheral plasma after administration of prostaglandin E 2 tablets used for induction of labor. Prostaglandins 13, 399-408. Granström, Ε. (1971). Metabolism of prostaglandin F 2 a in swine kidney. Biochim. Biophys. Acta 239, 120-125. , and Samuelsson, B. (1972). Development and mass spectrometric evaluation of a radioimmunoassay for 9α, 11a, dihydroxy-15-ketoprost-5-enoic acid. FEBS Lett. 26, 211-214.

And Rivetz, B. (1972). Preparation of specific antiserum to prostaglandin A. Prostaglandins 2, 103-113. Stylos, W. , Ritzi, Ε. , and Watson, D. J. (1973). A radioimmunoassay for the initial metabolites of the F prostaglandins. Prostaglandins 4, 553-565. 42 Bernard M. Jaffe and Harold R. Behrman Tashjian, A. , Voelkel, E. , and Levine, L. (1975). Hydrocortisone inhibits prostaglandin production by mouse fibrosarcoma cells. Nature (London) 258, 739-741. , and Horton, E. W. (1970). The separation, identification, and estimation of prostaglandins in nanogram quantities by combined gas chromatography-mass spectroscopy.

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