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Extra info for Methods in cell physiology. / Volume IV
3. Murray, M. , and Kopech, G. ( 1953). ” Academic Press, New York. Niu, M. , and Twitty, V. C. (1953). Proc. Natl. Acad. Sci. U. S . 39, 985-989. Parker, R. C. (1961). , Harper and Row, New York. Paul, J. (1965). , E. & S. , Edinburgh and London. Porter, K. R. (1939). Biol. Bull. 77, 233-257. Preston, M. M’E. (1949). J . Roy. Micro. SOC. 69, 65-71. Puck, T. , and Marcus, P. I. (1955). Proc. Natl. Acad. Sci. U. S . 41, 432437. Puck, T. , Marcus, P. I, and Cieciura, S. J. (1956). J . Exptl. Med.
A plate should be kept a minimum of 5-7 days. The delay is not serious at the two most crucial stages for sterility testing-cysts and very small cells. These can be held in the dark until the plate is read. If contamination is found, treatment can be given or the culture discarded. Routine platings of the medium supply and cell stocks are required. F. The Synthetic Medium The requirements of a suitable medium are: (1) that it can be autoclaved; ( 2 ) that it is chemically defined; ( 3 ) that it supports normal cell growth; and ( 4 ) that all cell nutrients must be in adequate supply to support cell growth from the negligible wet weight of the inoculum to the maximum weight that the CO, diffusion rate will permit under the standard culture conditions.
U. S . 39, 985-989. Parker, R. C. (1961). , Harper and Row, New York. Paul, J. (1965). , E. & S. , Edinburgh and London. Porter, K. R. (1939). Biol. Bull. 77, 233-257. Preston, M. M’E. (1949). J . Roy. Micro. SOC. 69, 65-71. Puck, T. , and Marcus, P. I. (1955). Proc. Natl. Acad. Sci. U. S . 41, 432437. Puck, T. , Marcus, P. I, and Cieciura, S. J. (1956). J . Exptl. Med. 103, 273-284. Rafferty, K. , Jr. (1965). Ann. N. Y. Acad. Sci. 126, 3-21. Rafferty, K. , Jr. ( 1970). In “Biology of Amphibian Tumors” ( M .