By Werner Klipp (Editor), Bernd Masepohl (Editor), John R. Gallon (Editor), William E. Newton (Editor)
This booklet presents a entire and specific resource of knowledge at the genetic and regulatory elements of organic nitrogen fixation in free-living (non-symbiotic) prokaryotes. organic nitrogen fixation is represented in a various diversity of microorganisms, between which Klebsiella pneumoniae serves as a paradigm for the genetic research of diazotrophy, that's the power to develop with N2 as sole nitrogen resource. the amount makes use of significant complementary ways to the subject material. The preliminary chapters use an organismic-based technique by means of targeting the well-characterized diazotrophic proteobacteria, cyanobacteria, Gram-positive clostridia, and Archea. The later chapters use a comparative process-based process and function overviews facing diverse regulatory features, electron delivery to nitrogenase, and molybdenum metabolism, around the variety of organisms. each time acceptable, old facets and agricultural and ecological affects were considered. every one bankruptcy includes an intensive record of references. This booklet is the self-contained moment quantity of a accomplished seven-volume sequence. No different to be had paintings offers the up to date and in-depth insurance of this sequence and this quantity. This e-book is meant to function an essential reference paintings for all scientists operating during this and heavily similar fields, to aid scholars to go into this demanding zone of study, and to supply technological know-how directors easy accessibility to important correct details.
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Additional resources for Genetics and Regulation of Nitrogen Fixation in Free-Living Bacteria (Nitrogen Fixation: Origins, Applications, and Research Progress)
2002) Direct interaction of the NifL regulatory protein with the GlnK signal transducer enables the Azotobacter vinelandii NifL-NifA regulatory system to respond to conditions replete for nitrogen. J. Biol. Chem. 277, 15472-15481. , van Heeswijk, W. , and Dixon, R. (2000) Signal transduction to the Azotobacter vinelandii NIFL-NIFA regulatory system is influenced directly by interaction with 2-oxoglutarate and the PII regulatory protein. , 19, 6041-6050. MacFarlane, S. , and Merrick, M. (1985).
In the absence of purified proteins, early studies on NifA-mediated transcription relied upon in vivo footprinting techniques. Dimethyl sulphate protection experiments indicated that V54-holoenzyme bound to the -24 –12 region of the promoter and sequences around the transcription site showed chemical reactivity to potassium permanganate when the promoter was activated by NifA in vivo. These experiments suggested that, like NtrC, NifA catalysed the isomerization of closed complexes formed with V54-RNA polymerase holoenzyme to open promoter complexes (Morett and Buck, 1989).
Ashby, G. , Drummond, M. , Eady, R. , et al. (1992). Posttranslational modification of Klebsiella pneumoniae flavodoxin by covalent attachment of coenzyme A, shown by 31P NMR and electrospray mass spectrometry, prevents electron transfer from the nifJ protein to nitrogenase. A possible new regulatory mechanism for biological nitrogen fixation. , 31, 1216-1224. Tubb, R. S. (1974). Glutamine synthetase and ammonium regulation in Klebsiella. Nature, 251, 481. , and Merrick, M. J. (1988). Over-production and characterisation of the nifA gene product of Klebsiella pneumoniae - the transcription activator of nif gene expression.