By William C. Heiser
Hugely skilled researchers describe in step by step element tools that experience confirmed most precious in offering genes to mammalian cells. quantity 1: Nonviral Gene move ideas makes a speciality of gene supply through quite a few chemical and actual tools, together with ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying quantity, quantity 2: Viral Gene move ideas, info approaches for providing genes to cells in vitro and in vivo, together with using lentiviral vectors.
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Extra info for Gene Delivery to Mammalian Cells Vol 1: Nonviral Gene Transfer Techniques (Methods in Molecular Biology Vol 245)
With the development of sensitive dioxetane substrate for alkaline phosphatase, the detection limit of SEAP is less than 10Ϫ18 mole of the enzyme. 6. Summary and Outlook The use of synthetic vectors for DNA delivery into mammalian cells in vitro is now a well-established technique. There is also a rapid increase in use of synthetic vectors for in vivo DNA delivery. Cationic liposomes have already been used in clinical trials (63–66). However, most of the in vivo applications are conducted through local regional administration.
Mack, K. , and McGrath, M. (1998) A novel method for DEAE-dextran mediated transfection of adherent primary cultured human macrophages. J. Immunol. Methods 211, 79–86. 7. Rupprecht, A. P. and Coleman, D. L. (1991) Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran. J. Immunol. Methods 144, 157–63. 8. Gauss, G. H. and Lieber, M. R. (1992) DEAE-dextran enhances electroporation of mammalian cells. Nucleic Acids Res. 20, 6739–6740. 9. Graham, F. L. and Van der Eb, A.
32 Pari and Xu References 1. , and Taniguchi, T. (1986) Regulation of human interleukin-2 gene: functional DNA sequences in the 5Ј flanking region for the gene expression in activated T lymphocytes. Cell 46, 401–407. 2. Lopata, M. , Cleveland, D. , and Sollner-Webb, B. (1984) High-level expression of a chloramphenicol acetyltransferase gene by DEAE-dextran-mediated DNA transfection coupled with a dimethysulfoxide or glycerol shock treatment. Nucleic Acids Res. 12, 5707–5711. 3. , and Howard, B.