Nonfiction 14

Enzymatic Synthesis of Structured Triglycerides: From by María Luján Ferreira, Gabriela Marta Tonetto

By María Luján Ferreira, Gabriela Marta Tonetto

This short offers the cutting-edge on enzymatic synthesis of established triglycerides and diglycerides, concentrating on glycerol because the substrate and overlaying interesterification of vegetable oils in a single and steps. It severely studies the to be had literature on enzymatic and chemo-enzymatic synthesis of di- and triglycerides in a single or extra steps. the results of the constitution, size and unsaturation of the fatty acids are rigorously thought of, in addition to the inhibitory capability of hugely unsaturated advanced fatty acid structures.

The short additionally addresses acyl migration and using adsorbents, bearing in mind the newest literature and proposing the matter in an business context. It discusses experimental and analytical difficulties touching on, e.g. the lab scale and the scaling as much as bench and pilot crops. numerous examples are offered, and their successes and screw ups are assessed. Biocatalysts in line with lipases are analyzed with reference to difficulties of immobilization, balance on garage time and task after a number of makes use of. the necessity for particular Sn-2 lipases is gifted and methods for optimizing Sn-2 esterification are mentioned. finally, useful features are tested, e.g. lipase “leaching” with lack of job, considering the most recent findings on non-stop and batch reactor configurations and providing the benefits and drawbacks of each.

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Extra resources for Enzymatic Synthesis of Structured Triglycerides: From Laboratory to Industry

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The preparation of cocoa butter equivalent (CBE) fats and human milk fat replacers are two of the most obvious examples. CBE and low calorie fats, together with MCTs, are three of the most important STs produced by different processes. 2 A  cidolysis of Vegetable/Edible Oils or Synthetic Triglycerides Even though there are many studies and reports of enzymatic acidolysis of vegetable oils (olive, sunflower, safflower, soy) [21, 22], with one or several fatty acids, there are many drawbacks of this route.

The reactants and the biocatalyst are placed in the tank and allowed to react. After completion of the reaction, the products and unreacted reagents are removed and the spent immobilized enzyme is separated from the reaction medium by filtration or centrifugation, and recovered to be used in subsequent batches. The biocatalyst is replaced when the enzymatic activity decreases. These kinds of reactors are versatile and easy to operate (cleaning, maintenance, temperature control). They are indicated for small-scale production of high-value compounds.

Following either a chemical or enzymatic process, it is necessary to separate the fatty acids, MGs and DGs. Fatty acids may be neutralized and the MGs removed by distillation. It is important to minimize production of DGs. 1 Chemical Interesterification Sodium methoxide or a similar material is generally used. The reaction is carried out at close to 90–120 °C under vacuum or under nitrogen. The product is a complex mixture with a complete randomization of the fatty acids across all glycerol positions.

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