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Extra resources for Electrophysiology - From Plants to Heart
3 pA at –60 mV. 5 Calcium currents After block of the TTX-sensitive component and suppression of the K-current by equimolar substitution of intracellular K+ with Cs+, a persistent inward current could be observed at the end of prolonged depolarising steps (Fig. 7A, upper trace). This residual fraction could be almost completely blocked by Cd2+ or Co2+ ions (Fig. 7A, lower traces), suggesting that this second component was sustained by calcium ions. The very small fraction of current remaining after TTX and Cd2+ block has not been further investigated in the present study.
4A). 3 M) at all voltages, and its abolition following removal of sodium ions from the perfusing medium indicate that it is a classical Na-current. Although it was not always possible to exert an accurate control of membrane potential during the transient sodium current in DA cells in slice preparations (presumably because of currents generated at a distance from the soma on the axon or dendrites), an adequate space clamp and series resistance compensation could be achieved in 7 neurones in which we could obtain a complete series of recordings with and without TTX.
Sinykhin, A. M. (1962). A spreading wave of excitation in higher plants. Proc. Academy Science USSR (Botany) 142: 214–215. Gurovich, L. (2009). Real-Time Plant Water Potential Assessment Based on Electrical Signaling In Higher Plants Joint International Meeting. VII. , paper N° 095875. Gurovich, L. & Hermosilla, P. (2009). Electric signaling in fruit trees in response to water applications and light-darkness conditions. J Plant Physiology 166:290–300. Haberlandt, G. (1914). Physiological plant anatomy.