By Claudio Nicolini
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Extra info for Chromatin Structure and Function: Molecular and Cellular Biophysical Methods
Olins, A. , Carlson, R. , and Olins, E. E. (1975) J. Cell BioI. 528-537. ~, Olins, A. , and Olins, D. E. (1974) Science 183, 330-332. Ottensmeyer, F. , Hhiting, R. , Schmidt, E. , and Clemens, R. S. (1975) J. Ultrastruct. Res. ~, 193-201. Pooley, A. , Pardon, J. , and Richards, B. M. (1974) J. Mol. BioI. ~, 533-549. Woodcock, C. L. , Safer, J. , and Stanchfield, J. E. (1976) Exp. Cell Res. 22, 101-110. ATIN R. Stewart Gilmour Beatson Institute for Cancer Research Wolfson Laboratory for Molecular Fathology Garscube Estate, Switchback Road, Bearsden Glase;ow, G6l JBD, Scotland WHAT IS CHROMATIN?
The rapid acceptance of the universality of the nucleosome as the fundamental unit of chromatin structure depended, in large measure, on its ubiquitous distribution in electron micrographs and its correlation with biochemical data. o 4. , 1974) and the appearance of ELECTRON MICROSCOPY FOR VISUALIZING CHROMATIN 35 nucleosomes in the electron microscope (\Voodcock et al. , 1976) • For this reason, critical-point drying, and dehydration with organic solvents, has been avoided in our laboratory. Salt and pH conditions must also be carefully controlled and their effects understood.
There seems to be some varianoe in the literature as to the amount of endogenous RNA found in the chromatin of different systems, as judged by control incubations containing chromatin but no polymerase. Nearly allthe data referred to are derived from hybridizations with excess RNA, where even minute levels of endogenous RNA contamination of the ~ ~ t~cripts will produce misl~ng results. It is not oertain that actively transcribing inoubations and oontrol incubations are exactly eqUivalent in all other respects except for the presence of RNA po~erase.