Techniques

Cerebral Blood Flow: Mathematical Models, Instrumentation, by Aldo Rescigno (auth.), Aldo Rescigno, Andrea Boicelli (eds.)

By Aldo Rescigno (auth.), Aldo Rescigno, Andrea Boicelli (eds.)

The NATO complicated learn Institute on "Cerebral Blood move: Mathematical types, Instrumentation, and Imaging innovations" was once held in L'Aquila, Italy, June 2-13, 1986. Contributions to this application have been bought from the college of L'Aquila, Consiglio Nazionale delle Ricerche, Siemens Elettra S.p.A., and Bracco S.p.A. fresh stories of the cerebral blood circulate have lagged at the back of research of different parameters comparable to glucose usage, transmitter distribution, and precursors. This complex examine Institute attempted to fill this hole via examining intimately diverse actual recommendations corresponding to Autoradiography (including Double-Tracer vehicle­ radiography and hugely particular tracers as Iodoantipyrine, Micro­ spheres), unmarried Photon Emission Computed Tomography, Nuclear Magnetic Resonance. every one technique used to be analyzed with reference to its precision, solution, reaction time. a substantial a part of this Institute was once dedicated to the math of CBF size, in its points, i.e. the modeling of the underlying kinetic process and the statistical research of the information. The modeling tools proposed integrated the advance of a differential algebra wherein the differential and quintessential equations concerned should be solved via basic algebraic equipment, together with graph­ theoretical ones; the statistical equipment proposed integrated the representation of other parametrizations of attainable use within the interpretation of experimental results.

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Additional resources for Cerebral Blood Flow: Mathematical Models, Instrumentation, and Imaging Techniques

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N 1 lr,l,n +,(t) J 1 for j = 2,···, n2 28 Letting H(t) = [w .. frix, it follows that the system of equations in Eq. (24) may be represented as W(t) where K W(t)K (25) -(k 10 +k 12 ) (k 10 +k 12 ) 0 -(k 10:k 12 ) 0 o. 0 0 O. 0 0 o. (k 10 +k 12 ) O. 0 0 O. 0 0 0 0 -(k 20:k 12 ) 0 0 o. -(k 21 +k 21 ) J \ n 1 columns ~ n 2 columns The general solution to Eq. (25) is W(t) (26) as before in Eq. (14), however, the eigenvalues in the nontrivial case n 1 + n2 > 2 are not distinct and not necessarily real; hence the Wij(t), and subsequent p ..

Continued) 39 tissues only a very small fraction of the deoxyglucose-6-phosphate formed proceeds to these products (22). In any case, these compounds are secondary, relatively stable products of deoxyglucose-6-phosphate, and all combined together represent the products of deoxyglucose phosphorylation. Deoxyglucose-6-phosphate and its derivatives, once formed, are, therefore, essentially trapped in the cerebral tissues, not forever but long enough to allow a reasonable period of measurement. , less than one hour) to allow the assumption of negligible loss of [14 c JDG-6-P and/or its secondary products from the tissues, then the quantity of labeled products accumulated in any cerebral tissue at any given time following the introduction of [14CJDG into the circulation is equal to the integral of the rate of [14CJDG phosphorylation by hexokinase in that tissue during that interval of time.

The brain sections are picked up on gl ass cover slips, dried on a hot plate at 60°C for at least 5 minutes, and placed sequentially in an X-ray cassette. , Arlington Heights, IL), which include a blank and a series of progressively increasing 14C concentrations, are also placed in the cassette. These standards must previously have been calibrated for their autoradiographic equivalence to the 14C concentrations in brain sections, 20 ~m in thickness, prepared as described above. The method of calibration has been previously described (29).

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